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Fastsybr mixture

WebJul 28, 2024 · HIF-1α mRNA quantification was performed using 2X SGExcel FastSYBR Mixture (Sangon Biotech Co., Ltd.). The RNA was reverse transcribed using the oligo (dT) 15 primer. A total of 500 ng RNA, 20 pmol oligo (dT) 15 primer, 4 µl 5X reaction buffer, 0.5 µl Ribolock RNase inhibitor, 2 µl dNTP mix and 1 µl RevertAid Reverse Transcriptase were ... Web提供亚洲棉和雷蒙德氏棉PEBP家族基因的鉴定及该家族基因在陆地棉组织中表达分析文档免费下载，摘要:396作物学报第41卷叶片将幼苗顶端分生组织分离出来;采集开花当天的花、胚珠以及25DPA(daypost-anthesis)的棉桃,将纤维从棉桃中剥离。将所有棉花组织立即浸没于液氮中,–80℃保存备用。

Fast SYBR® Green Master Mix Thermo Fisher Scientific

WebFast SYBR Green Master Mix contains everything you need, excluding the template and primers, in a convenient 2X mix. It includes the following components in an optimized … WebFeb 27, 2024 · In this paper, a fast and simple strategy for sensitive detection of streptavidin (SA) was proposed based on terminal protection of small molecule-linked DNA and cationic conjugated polymer-mediated fluorescence resonance energy transfer (FRET). In principle, we designed a biotin-labelled DNA probe (P1) as the recognitive probe of SA, along with … times of india paper subscription offer https://bioforcene.com

FastStart™ 通用SYBR® Green预混液(ROX) Sigma-Aldrich

WebFastSYBR Mixture是专用于染料法 (SYBR GreenⅠ)实时荧光定量PCR的预混体系，浓度为2×,包含Fast Taq DNA Polymerase、PCR Buffer、dNTPs、SYBR Green I荧光染料 … WebOct 10, 2016 · The mixture was stirred for 24 h at RT followed by purification using 10,000 MWCO centrifugal filters with HBS. The concentration of HAI peptide in the conjugate was measured spectrophotometrically at 280 nm. The concentration of bPEI in the conjugate was determined by a TNBS assay . Briefly, the according amount of HAI peptide was added … WebThe UltraSYBR Mixture (High ROX) is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2x. It contains GoldStar Taq … times of india paper rate

Neohesperidin enhances PGC-1α-mediated mitochondrial

Category:Epithelial NELF guards intestinal barrier function to ameliorate ...

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Fastsybr mixture

Roche罗氏04913914001 FastStart™ 通用SYBR® Green预混液(ROX)

WebFastStart ™ Universal SYBR ® Green预混液 (Rox)是一种用于qPCR和RT-qPCR的即用型热启动反应混合物，适用于所有需要用ROX标准化的实时荧光定量PCR系统。 SYBR ® … WebThen, the expression of circRNAs was assessed using RT-PCR and the SGExcel FastSYBR Mixture (with ROX) (Sangon Biotech) in an ABI 7500 system (Applied Biosystems, CA, USA). Ten predicted miRNAs were reverse transcribed into cDNA using an miRNA First Strand cDNA Synthesis kit (Tailing Reaction) (Sangon Biotech).

Fastsybr mixture

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WebOct 28, 2024 · Real-time PCR was performed using one SGExcel FastSYBR qPCR Mixture (Sangon Biotech, Shanghai, China) on an ABI One Step Plus PCR System (Applied Biosystems, Foster City, CA, USA). Each reaction contained 10 µL SGExcel FastSYBR qPCR Mixture, 0.2 µM primer, and 1 µL template cDNA. The PCR reaction parameters … WebThe Fast SYBR® Green Cells-to-CT™ Kit includes reagents and enzyme mixtures for reverse transcription (RT) and real-time PCR directly from cultured cell lysates, without …

WebMar 1, 2024 · SGExcel FastSYBR Mixture (SYBR Green I), amino-DNA and primers were purchased from Shanghai Sangon Biotech, China. The sequences of the amino-DNA … WebOct 25, 2024 · The abundances of specific intestinal groups were determined by qPCR with FastSYBR mixture (CWBIO, China) and conserved specific 16S rDNA primers . The intestinal microbiota 16S rDNA sequencing and analysis were performed as previously described 49,51. Briefly, the total bacteria 16S rDNA of distinct regions (16S V4) were …

WebThe FastSYBR Mixture is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the concentration is 2×. It contains GoldStar Taq DNA Polymerase, PCR Buffer, dNTPs, SYBR Green I Fluorescent Dye, and Mg2+. The operation is simple and convenient. This product is mainly used for the detection of genomic DNA target ... Web阿里巴巴Roche罗氏04913914001 FastStart™ 通用SYBR® Green预混液(ROX)，生化试剂，这里云集了众多的供应商，采购商，制造商。这是Roche罗氏04913914001 FastStart™ 通用SYBR® Green预混液(ROX)的详细页面。产地:Roche罗氏，型号:04913914001，是否进口:否，分类:预混液，货号:04913914001，级别:优级纯GR，含量:咨询 ...

WebFastsybr Green Pcr Master Mix Qiagen Bioz Bioz Stars score, Techniques, Protocol Conditions and more for Fastsybr Green Pcr Master Mix, supplied by Qiagen. Data for …

Webvolumes comprising 5 µl 2× FastSYBR Mixture (CWbiotech, China), 0.5 µl primer mixture (final concentration of each primer 0.25 pmol), 1 µl DNA template with different amounts of DNA, and 3.5 µl ddH 2 O. Data were collected using an ABI analytical thermal cycler. RNA expression was calculated based on a relative standard times of india paper priceWebFastsybr Green Pcr Master Mix Qiagen Bioz Bioz Stars score, Techniques, Protocol Conditions and more for Fastsybr Green Pcr Master Mix, supplied by Qiagen. Data for Fastsybr Green Pcr Master Mix gathered from related PubMed articles. Home > Search Results > Qiagen > fastsybr green pcr master mix Similar Products parenting is not easyWebJul 18, 2024 · FastSYBR Mixture Catalog Number: CW0955S 1ml, CW0955M 5 ml, CW0955L 25 ml Storage Condition: -20°C; if used frequently, store at 2-8°C to avoid repeated freezing and thawing. Product Introduction: The FastSYBR Mixture is a premixed system for real-time fluorescence quantitative PCR (SYBR Green I), and the … times of india parenting