WebMay 14, 2024 · To monitor GBP2/5 expression in primary cells, we first validated the specificity of GBP2 and GBP5 antibodies using HEK293T cells transfected with hemagglutinin (HA)-tagged versions of GBP1–6 (Figure S2 A).Western blot and/or flow cytometric analyses of primary human monocyte-derived macrophages (MDMs) and … WebAug 11, 2024 · Guanylate binding protein 5 (GBP5) is the interferon (IFN)-inducible subfamily of guanosine triphosphatases (GTPases) and is involved in pathogen defense. …
Guanylate-binding proteins promote activation of the AIM2
WebMar 29, 2024 · New research led by scientists at Washington State University has found that a protein known as GBP5 appears to play a key role in suppressing inflammation in rheumatoid arthritis, a... WebMar 16, 2015 · How host cells induce the release of DNA from cytosolic bacteria is unclear. Broz and colleagues show that the guanylate-binding proteins GBP2 and GBP5 trigger bacteriolysis in infected host cells ... cooling suit for motorcycle
Whole blood GBP5 protein levels in patients with and …
WebApr 22, 2024 · The GBP5 protein demonstrates anti-inflammatory properties in other immune cell types that play important roles in the development of rheumatoid … WebFeb 19, 2024 · We also examined the protein levels of GBP5 in a collection of 12 glioma cell lines. It was found that the expression levels of GBP5a/b were increased in 5 of 12 (41.67%) ... WebDec 27, 2024 · Gbp5 mRNA and protein levels were measured by qPCR and Western blot (n = 3–6). (H) Primary hepatocytes were treated with TNFα (10 ng/ml), IFNγ (100 ng/ml), and TNFα plus IFNγ for 12 h, respectively. Gbp5 mRNA and protein levels were measured by qPCR and Western blot. *p < .05. **p < .01. Data represent the mean ± SEM cooling suits for racing