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Hieffclone

Web12 de nov. de 2024 · 一种pbiflu荧光标签表达载体的制备方法及其应用 技术领域 1.本发明属于分子生物学技术领域，具体涉及一种pbiflu荧光标签表达载体的制备方法及其应用。 背景技术： 2.黄色荧光蛋白(yellow fluorescent protein，yfp)是将绿色荧光蛋白(gfp)第203为苏氨酸突变体为色氨酸，可以稳定生色团在激发状态的偶极矩 ... Web10 de abr. de 2024 · Hieff CloneTM Plus One Step Cloning Kit是一款简单、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任意载体的任意位点，可高效 …

Anti-Restriction Protein, KlcA HS , Promotes Dissemination of ...

WebCN114134170A CN202411457529.2A CN202411457529A CN114134170A CN 114134170 A CN114134170 A CN 114134170A CN 202411457529 A CN202411457529 A CN 202411457529A CN 114134170 A CN114134170 A CN 114134170A Authority CN China Prior art keywords mcs product pgl primer nos Prior art date 2024-12-02 Legal status … Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID fragment was amplified by PCR from a commercial gene (USA126049; Addgene, Watertown, MA, USA). The sequences of the primers were listed in Table S1. how to serve tinga https://bioforcene.com

(PDF) Upregulation of PAIP1 promotes the gallbladder …

Web产品描述. 本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成 … Web1 de jun. de 2024 · this digested vector by a HieffClone Plus multi One-Step . Cloning Kit (10912ES10, Y esen, China). The T urboID . fragment was amplified by PCR from a commercial gene Web1 de dez. de 2024 · The enzymes using in molecular cloning experiment were Sap Ⅰ (NEB, USA), T4 DNA Ligase (Takara, Japan) and 2 × Hieffclone Enzyme Mix (YEASEN, … how to serve tennis tips

Gene editing of Duchenne muscular dystrophy using

Category:Frontiers gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and ...

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Hieffclone

HieffClone®ZeroTOPO-TASimpleCloning Kit,MCS-Depleted

Web1 de dez. de 2024 · Introduction. Mutations Duchenne muscular dystrophy (DMD) is hereditary disease carried by the X chromosome, which driven by the mutation in DMD gene [1,2].DMD gene encodes Dystrophin protein that maintains to support and stabilize the structure and function of muscle tissue. The mutation of DMD gene lead to an absence … WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB

Hieffclone

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Web公司简介. 上海翊圣生物科技有限公司成立于2009年，总部设在上海，并且分别在北京、杭州、南京、武汉、广州（深圳）设有办事处。. 公司专注于酶制试剂和抗原抗体的开 … Web15 de dez. de 2024 · Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of …

Web2 de mai. de 2024 · Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the …

Web本试剂盒是利用拓扑异构酶 (Topoisomerase)高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需 1-5 min即可完成连接反应。. … Web16 de dez. de 2024 · The linear DNA was cyclized into ΔKlcA HS pHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) …

Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID …

WebHieff Clone™One. 收藏. 价格 ￥1 - 1680. 产地 德国/中国. 货号 10905ES25. 品牌 进口/国产. 技术资料 免费索取技术资料. 丁香通用户服务中心 查看详情. 在线咨询. how to serve trifleWeb丁香通为您提供HieffClone™P商品详情介绍：价格：￥410，货号：10912ES，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone™P商品详情页； 丁香通 丁香园 how to serve thin sliced smoked salmonWeb6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in the transformed E. coli by recombination according to HieffClone TM MultiOneStep Cloning kit (YEASEN, Shanghai, China). how to serve wagyu beefWeb15 de dez. de 2024 · 上海翊圣生物科技有限公司怎么样？企查猫(企业查询宝)为您提供上海翊圣生物科技有限公司的工商注册信息、纳税人识别号、公司地址、公司电话、商标专利、最新招聘等，让您全方位了解上海翊圣生物科技有限公司的信用信息，一键快速摸底企业。 how to serve underserved communitieshttp://www.labbase.net/Supply/SupplyItems-4428942.html how to serve turnipsWebHieffClone®ZeroTOPO-TASimpleCloningKit不含多克隆酶切位点(MCS)，在引入后续酶切位点进行酶切操作时，不会受到 MCS位点的影响，从而增加酶切效率以及克隆成功率。 … how to serve tuacaWeb20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, … how to serve thomas dakin gin