Web1 Oct 2014 · Serine/threonine ligation has been developed for the chemical synthesis of proteins. • Ser/Thr ligation-mediated cyclization can be used for cyclic peptides synthesis. … Web23 Apr 2013 · The utility of this peptide ligation approach has been demonstrated through the convergent syntheses of two therapeutic peptides--ovine-corticoliberin and Forteo--and the human erythrocyte acylphosphatase protein (∼11 kDa).

A study on L-threonine and L-serine uptake in Escherichia coli K-12

Web22 Oct 2013 · The 5-lysyl-hydroxylation has been found among protein post-translational modifications. In this report, we have devised a fluorescence turn-on probe which allows for selectively recognizing 5-hydroxylysine-containing peptides. This article is part of the themed collection: 2014 Emerging Investigators Supplementary information WebFinally, the review focuses on the discovery and development of peptide ligation and the latest advances in this field including native amide bond formation strategies, these include the native chemical ligation, ±-ketoacid-hydroxylamine ligation, and serine/threonine ligation which are the most commonly used chemoselective ligation methods ... how to use trade credit gamestop online

A study on L-threonine and L-serine uptake in Escherichia coli K-12

WebMore importantly, several important enzymes and transcription factors involved in chilling injury, such as ATPase, serine/threonine protein kinase, β-galactosidase, heat shock protein, ethylene-responsive transcription factor, were found in the ceRNA network in our results, indicating their specific cooperative regulation roles in chilling stress . In addition, the … WebTo allow for the multiple-fragment ligation, we chose the Msz (p-(methylsulfinyl)benzyloxycarbonyl) group, which is compatible with the preparation of … WebThe ligation mixture was electroporated into XL1 Blue cells. The cells were resuspended in 200 ml LB medium containing 200 mg/l ampicillin and grown to saturation. ... Further work is thus needed to test these hypotheses and to explore the response of the serine/threonine transport subsystem to specific environmental conditions. how to use trade me