Webb22 maj 2024 · Chemical probing is an important tool for characterizing the complex folded structures of RNA molecules, many of which play key cellular roles. Electrophilic SHAPE reagents create adducts at... WebbSHAPE chemistry exploits the reactivity of the RNA ribose 2′-OH group using small hydroxyl-selective electrophilic reagents. In contrast to other chemical probing techniques, SHAPE reactivities detect and quantify local nucleotide flexibility and dynamics, ignoring base identity [23] [24] [25] [26] [27] [28] [29] [30] .

RNAProbe :: About SHAPE method

WebbAccess Provided by Peking University JOURNALS A-Z WebbSHAPE probing allows for studying the flexibility of RNA backbone, based on the susceptibility of nucleotides to chemical modification. RNA-modifying agents, such as … cs400b sh401ba

Technique Development for Probing RNA Structure In Vivo and …

WebbRNA structure analysis by selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) takes advantage of the reactivity of small electrophilic chemical probes that react with the 2′-hydroxyl group to assess RNA structure at nucleotide resolution. Webb20 maj 2015 · The SHAPE structure probing approach [ 7, 8] interrogates virtually all nucleotides of any RNA target. The adaptation of RNA chemical probing approaches to readout by massively parallel sequencing allows for high-throughput analysis and is rapidly advancing toward transcriptome-scale assays [ 9, 10 ]. WebbThe study of these bubbles is useful in determining the elastic and mechanical properties of graphene and adhesion energy between graphene and the substrate, and … cs401as