The phic31 integrase system for gene therapy
WebbThe integrase-mediated approach for gene knock-out (IMAGO) technique combines ends-out targeting with phiC31-mediated recombinase-mediated cassette exchange (RMCE) . IMAGO uses ends-out to replace the targeted locus with an attP-flanked selectable marker, which can subsequently be replaced with any desirable construct, thus enabling in situ … WebbThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA …
The phic31 integrase system for gene therapy
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Webb4 mars 2016 · The first studies utilizing phiC31 integrase for gene therapy took advantage of a relatively simple and effective in vivo delivery method in mice, hydrodynamic … WebbLieu et al. 1208 www.sbsonline.org Journal of Biomolecular Screening 14(10); 2009 after integration.12,13 PhiC31 integrase has been shown to target pseudo attP sites present …
WebbThe phiC31 system has been used to genetically engineer Drosophila and Xenopus and for in vivo and ex vivo gene therapy in several tissues in model organisms including mouse, … WebbINTRODUCTION: The phiC31 integrase system is a phage-derived system that offers the ability to integrate plasmid DNA into the chromosomes at a subset of endogenous …
WebbThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. Nucleic Acid Introduction Reagents … WebbThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide …
WebbThe potential use of the φC31 integrase system in gene therapy opens up the possibilities of new treatments for old diseases. φC31 integrase mediates the integration of plasmid …
ontario mass termination rulesWebbThe potential use of the fC31 integrase system in gene therapy opens up the possibilities of new treatments for old diseases. fC31 integrase mediates the integration of plasmid … ontario masonry restorationWebb28 feb. 2012 · In order to select relevant systems to evaluate new therapies, ... Gene therapy for cystic fibrosis: an example for lung gene therapy. Gene Ther. 2004; 11 suppl. … ione wa weather forecastWebbThe phiC31 integrase may represent one of the best hopes for nonviral gene therapies for RA. This article provides evidence that phiC31 integrase generates stable transgene expression in cultured synoviocytes and rabbit joints, illustrating the potential of this nonviral vector to be used for RA gene therapy. 9. ione wa transfer stationWebb1 jan. 2009 · These mutant integrases are useful for gene therapy and genome modification, and they demonstrate the feasibility of engineering φC31 integrase toward … ione wa to newport waWebb25 nov. 2024 · C. elegans benefits from a large set of tools for genome manipulation. Yet, the insertion of large DNA constructs and the generation of inversions is still … ontario marriages records onlineWebb11 apr. 2024 · Preprint ione wa train ride